Analytical Data
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Gene name
Ephrin-A1/EFNA1
- Application
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Biological Activity
1.Immobilized Human Ephrin-A1-His at 10 μg/mL (100 μl/well) can bind Human EphA2-Fc.The ED50 for this effect is 12.71 ng/mL. 2.Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) EphA2 is coated at 2 μg/mL (100 μL/well), the concentration of rhEphrin-A1 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 4.998 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) EphA2 is coated at 2 μg/mL (100 μL/well), the concentration of rhEphrin-A1 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 4.998 ng/mL.
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Alternative Names
Interferon lambda-3; IFN-lambda-3; IL-28B; IL-28C; ZCYTO22
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
NP_004419.2
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Expression Region
D19-S182
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Protein Length
Partial
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Molecular Weight
25-30 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process











