Analytical Data
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Gene name
HNRNPH3
- Application
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Alternative Names
HNRNPH3; HNRPH3; Heterogeneous nuclear ribonucleoprotein H3; hnRNP H3; Heterogeneous nuclear ribonucleoprotein 2H9; hnRNP 2H9
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31942
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Expression Region
1-331aa
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AA Sequence
MDWVMKHNGPNDASDGTVRLRGLPFGCSKEEIVQFFQGLEIVPNGITLTMDYQGRSTGEAFVQFASKEIAENALGKHKERIGHRYIEIFRSSRSEIKGFYDPPRRLLGQRPGPYDRPIGGRGGYYGAGRGSYGGFDDYGGYNNYGYGNDGFDDRMRDGRGMGGHGYGGAGDASSGFHGGHFVHMRGLPFRATENDIANFFSPLNPIRVHIDIGADGRATGEADVEFVTHEDAVAAMSKDKNNMQHRYIELFLNSTPGGGSGMGGSGMGGYGRDGMDNQGGYGSVGRMGMGNNYSGGYGTPDGLGGYGRGGGGSGGYYGQGGMSGGGWRGMY
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Molecular Weight
61.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HNRNPH3, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, plays a crucial role in various cellular processes, including pre-mRNA splicing, mRNA stability, and transport. Research into HNRNPH3 has gained prominence due to its implications in gene expression regulation and potential involvement in various diseases, including cancer and neurodegenerative disorders. This protein is known to bind to RNA and influence the alternative splicing of pre-mRNAs, which affects the diversity of protein isoforms generated in cells. Studies have also indicated that HNRNPH3 may have a role in the cellular response to stress, thereby impacting cell survival and apoptosis. The reconstitution of HNRNPH3 as a recombinant protein is essential for elucidating its structure-function relationship, allowing researchers to investigate its molecular mechanisms and interactions with RNA and other proteins. Understanding HNRNPH3's precise functions may provide insights into its potential as a therapeutic target, leading to the development of novel treatments for diseases associated with its dysregulation. Consequently, producing HNRNPH3 in a recombinant form could facilitate high-throughput functional assays and structural studies, thereby enhancing our understanding of its biological significance and roles in health and disease.
