Analytical Data
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基因名
FTO
- Application
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别名
FTO；KIAA1752；Alpha-ketoglutarate-dependent dioxygenase FTO
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9C0B1
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表达区间
32-505aa
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氨基酸序列
TPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRD LVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAE IAAACETFLKLNDYLQIETIQALEELAAKEKANEDAVPLCMSADFPRVGM GSSYNGQDEVDIKSRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWH HDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDIWHVGFKISWDIE TPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTGTL DYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQ FWFQGNRYRKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQR NEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDA SMPLPFDLTDIVSELRGQLLEAKP
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分子量
56 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FTO (Fat mass and obesity-associated protein) is a well-studied gene that encodes a member of the α-ketoglutarate-dependent dioxygenase family, playing a significant role in the regulation of energy balance, body weight, and metabolism. Initially identified as a susceptibility gene for obesity, FTO has garnered attention due to its nuanced involvement in various biological processes, including RNA metabolism and epigenetic regulation. Recent research suggests that FTO preferentially demethylates N6-methyladenosine (m6A) modifications on RNA, influencing mRNA stability and translation. The protein's function in energy metabolism and its interactions within metabolic pathways have made FTO a target of interest for potential therapeutic interventions in obesity and related metabolic disorders. Additionally, FTO's diverse roles span cellular processes such as adipogenesis and neurogenesis, indicating its significance beyond mere obesity regulation. As research progresses, the recombinant expression of FTO protein allows for detailed structural and functional studies, essential for elucidating its mechanisms of action and interactions. Investigating FTO through recombinant technologies holds promise for the development of novel strategies in managing obesity, enhancing our understanding of metabolic syndrome, and addressing the global health challenges associated with these conditions.
