Analytical Data
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Gene name
FTO
- Application
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Alternative Names
FTO；KIAA1752；Alpha-ketoglutarate-dependent dioxygenase FTO
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9C0B1
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Expression Region
32-505aa
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AA Sequence
TPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRD LVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAE IAAACETFLKLNDYLQIETIQALEELAAKEKANEDAVPLCMSADFPRVGM GSSYNGQDEVDIKSRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWH HDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDIWHVGFKISWDIE TPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTGTL DYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQ FWFQGNRYRKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQR NEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDA SMPLPFDLTDIVSELRGQLLEAKP
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Molecular Weight
56 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FTO (Fat mass and obesity-associated protein) is a well-studied gene that encodes a member of the α-ketoglutarate-dependent dioxygenase family, playing a significant role in the regulation of energy balance, body weight, and metabolism. Initially identified as a susceptibility gene for obesity, FTO has garnered attention due to its nuanced involvement in various biological processes, including RNA metabolism and epigenetic regulation. Recent research suggests that FTO preferentially demethylates N6-methyladenosine (m6A) modifications on RNA, influencing mRNA stability and translation. The protein's function in energy metabolism and its interactions within metabolic pathways have made FTO a target of interest for potential therapeutic interventions in obesity and related metabolic disorders. Additionally, FTO's diverse roles span cellular processes such as adipogenesis and neurogenesis, indicating its significance beyond mere obesity regulation. As research progresses, the recombinant expression of FTO protein allows for detailed structural and functional studies, essential for elucidating its mechanisms of action and interactions. Investigating FTO through recombinant technologies holds promise for the development of novel strategies in managing obesity, enhancing our understanding of metabolic syndrome, and addressing the global health challenges associated with these conditions.
