Analytical Data
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Gene name
HDAC7A
- Application
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Alternative Names
DKFZP586J0917; FLJ99588; HD 7a; HD7; HD7a; HDAC 7; HDAC 7A; Hdac7; HDAC7_HUMAN; HDAC7A; Histone deacetylase 7; Histone deacetylase 7A; OTTHUMP00000202813; OTTHUMP00000202814
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WUI4
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Expression Region
645-915aa
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AA Sequence
VTDLAFKVASRELKNGFAVVRPPGHHADHSTAMGFCFFNSVAIACRQLQQQSKASKILIVDWDVHHGNGTQQTFYQDPSVLYISLHRHDDGNFFPGSGAVDEVGAGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVMPIAREFSPDLVLVSAGFDAAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASEACVAALLGNRVDPLSEEGWKQKPNLNAIRSLEAVIRVHSKYWGCMQRLASCPDSWVPRVPGA
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Molecular Weight
33.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Histone deacetylases (HDACs) play crucial roles in regulating gene expression, protein function, and cellular processes by removing acetyl groups from histone and non-histone proteins. Among the 18 known HDACs, HDAC7A (also referred to as HDAC7) has garnered significant attention due to its involvement in various biological processes and diseases, including cancer, cardiovascular disorders, and inflammatory responses. HDAC7A is primarily localized in the nucleus and is known to interact with several transcription factors, influencing the expression of key genes involved in cell proliferation and survival. The study of HDAC7A, particularly through recombinant protein techniques, has provided insights into its enzymatic function, substrate specificity, and regulatory mechanisms. Research on HDAC7A has highlighted its dual role in both promoting and inhibiting transcription, depending on the context and associated cofactors. Furthermore, the aberrant expression or activity of HDAC7A has been linked to tumorigenesis, making it a potential therapeutic target. The generation of recombinant HDAC7A allows for the detailed characterization of its biochemical properties, the screening of small molecule inhibitors, and the exploration of its role in signal transduction pathways. Thus, understanding the function and regulation of HDAC7A is essential for developing targeted strategies in treating diseases where its dysregulation is implicated. The ongoing research aims to unravel the complexities of HDAC7A's role in cellular regulation and its potential as a target for therapeutic intervention.
