Analytical Data
-
Gene name
GUCY1B2
- Application
-
Alternative Names
GUCY1B2; Guanylate cyclase soluble subunit beta-2; GCS-beta-2; EC 4.6.1.2
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O75343
-
Expression Region
1-617aa
-
AA Sequence
MSGYDRMLRT LGGNLMEFIE NLDALHSYLA LSYQEMNAPS FRVERGADGK MFLHYYSDRS GLCHIVPGII EAVAKDFFDI DVIMDILDMN EEVERTGKKE HVVFLIVQKA HRKMRKTKPK RLQDSQGMER DQEALQAAFL KMKEKYLNVS ACPVKKSHWD VVRSIVMFGK GHLMNTFEPI YPERLWIEEK TFCNAFPFHI VFDESLQVKQ ARVNIQKYVP GLQTQNIQLD EYFSIIHPQV TFNIFSIRRF INSQFVLKTR REMMPVAWQS RTTLKLQGQM IWMESMWCMV YLCSPKLRSL QELEELNMHL SDIAPNDTTR DLILLNQQRL AEIELSNQLE RKKEELQVLS KHLAIEKKKT ETLLYAMLPK HVANQLREGK KVAAGEFKSC TILFSDVVTF TNICTACEPI QIVNVLNSMY SKFDRLTSVH AVYKVETIGD AYMVVGGVPV PIGNHAQRVA NFALGMRISA KEVTNPVTGE PIQLRVGIHT GPVLADVVGD KMPRYCLFGD TVNTASRMES HGLPNKVHLS PTAYRALKNQ GFKIIERGEI EVKGKGRMTT YFLIQNLNAT EDEIMGRSKT PVDHKGSTQK ASLPTTKLQG SVQPSCPEHS SLASWLL
-
Molecular Weight
70.3 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GUCY1B2, a gene encoding the beta subunit of soluble guanylate cyclase (sGC), has gained attention in recent years due to its vital role in the nitric oxide (NO) signaling pathway, which is fundamental for various physiological processes, including vasodilation and neurotransmission. Dysfunction or mutations in GUCY1B2 have been implicated in cardiovascular diseases, making it a potential target for therapeutic intervention. Research has focused on recombinant GUCY1B2 protein for both structural and functional studies, providing insights into its interactions with nitric oxide and the regulatory mechanisms of sGC. Recombinant expression systems, including bacteria and yeast, allow for the production of this protein in sufficient quantities for detailed biochemical characterization and drug discovery applications. Furthermore, the exploration of GUCY1B2's response to different pharmacological agents could unveil new treatment strategies for conditions linked to NO signaling dysregulation, enhancing our understanding of cardiovascular and neurological disorders. Overall, the study of GUCY1B2 and its recombinant protein holds promise for advancing therapeutic developments aimed at modulating the NO-sGC signaling pathway.
