Analytical Data
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Gene name
GUCA1B
- Application
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Alternative Names
GCAP 2; Guanylate Cyclase Activating Protein Photoreceptor 2; Guanylate cyclase activator 1B; Guanylin 2; Guanylyl cyclase-activating protein 2; GUC1B_HUMAN; GUCA 2; Guca1b
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UMX6
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Expression Region
2-200aa
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AA Sequence
GQEFSWEEA EAAGEIDVAE LQEWYKKFVM ECPSGTLFMH EFKRFFKVTD DEEASQYVEG MFRAFDKNGD NTIDFLEYVA ALNLVLRGTL EHKLKWTFKI YDKDGNGCID RLELLNIVEG IYQLKKACRR ELQTEQGQLL TPEEVVDRIF LLVDENGDGQ LSLNEFVEGA RRDKWVMKML QMDMNPSSWL AQQRRKSAMF
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Molecular Weight
23.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GUCA1B encodes a subunit of the guanylate cyclase activated by calcium and calmodulin, playing a crucial role in phototransduction in retinal photoreceptors. Mutations in the GUCA1B gene have been associated with various retinal degenerative diseases, including retinitis pigmentosa and cone-rod dystrophy, leading to progressive vision loss. Research on GUCA1B recombinant proteins focuses on understanding its functional properties, interactions, and regulatory mechanisms within the phototransduction cascade. By producing and characterizing these recombinant proteins, scientists aim to elucidate how alterations in GUCA1B contribute to retinal pathologies. Furthermore, these studies also seek to explore potential therapeutic approaches, such as gene therapy or pharmacological interventions, that could restore or enhance the function of affected pathways in patients with GUCA1B-related retinal diseases. The investigation of GUCA1B recombinant proteins is pivotal not only for comprehending the underlying molecular mechanisms of sight restoration but also for advancing the development of novel treatments for genetic retinal disorders.
