Analytical Data
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基因名
IL-8/CXCL8
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简介
IL-8/CXCL8 protein, a vital chemotactic factor, orchestrates inflammatory responses by attracting neutrophils, basophils, and T-cells to clear pathogens. It activates neutrophils and binds to CXCR1/CXCR2 receptors, initiating downstream signaling pathways. IL-8/CXCL8 homodimerizes, disrupted by tick evasin-3, and interacts with TNFAIP6, potentially regulating chemokine activity in the inflammatory microenvironment. IL-8/CXCL8 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-8/CXCL8 protein, expressed by HEK293 , with N-His labeled tag.
- Application
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生物活性
Immobilized Human IL-8 at 1 μg/mL (100 μL/well) can bind Anti-IL-8 Antibody, The ED50 for this effect is 2.786 ng/mL. Immobilized Human IL-8 at 1 μg/mL (100 μL/well) can bind Anti-IL-8 Antibody, The ED50 for this effect is 2.786 ng/mL.
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别名
CXCL8; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1
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种属
Cynomolgus
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表达系统
HEK293
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标签
N-6*His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
XP_005555144.1
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表达区间
E21-P101
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蛋白长度
Partial
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分子量
11 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that plays a crucial role in the immune response, particularly in the recruitment and activation of neutrophils during inflammation. Research has increasingly focused on the functional implications of IL-8 in various pathological conditions, including autoimmune diseases, cancer, and chronic inflammatory disorders. Elevated levels of IL-8 have been associated with tumor progression and metastasis, making it a potential target for therapeutic interventions. Furthermore, the study of recombinant IL-8 protein is vital for understanding its precise biological functions and signaling mechanisms. Recombinant IL-8 can be utilized in various experimental applications, including cell migration assays, receptor binding studies, and the exploration of downstream signaling pathways. This protein is also of interest in vaccine development, as it may enhance immune responses. Given the significance of IL-8 in both health and disease, ongoing research aims to elucidate its complex role in inflammation and pathology, paving the way for potential therapeutic strategies that modulate IL-8 activity to improve patient outcomes.
