Analytical Data
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Gene name
IL-8/CXCL8
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简介
Interleukin-8 (IL-8), also known as CXCL8 or NAP-1, is a pro-inflammatory CXC chemokine. IL-8 acts on human neutrophils via two receptors, CXCR1 and CXCR2. IL-8 has a conserved Glu-Leu-Arg (ELR) N-terminal motif, and is an agonist for CXCR1/CXCR2. IL-8 is produced by various cells including leukocytes, endothelial cells, and epithelial cells[1][2][3]. IL-8/CXCL8 Protein, Human (77a.a, HEK293) is produced in HEK293 cells, and consists of 77 amino acids (A23-S99).
- Application
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Alternative Names
C-X-C motif chemokine 8; MDNCF; Emoctakin; NAP-1
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Species
Human
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10145
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Expression Region
S28-S99
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Protein Length
Partial
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Molecular Weight
11-13 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that plays a crucial role in the immune response, particularly in the recruitment and activation of neutrophils during inflammation. Research has increasingly focused on the functional implications of IL-8 in various pathological conditions, including autoimmune diseases, cancer, and chronic inflammatory disorders. Elevated levels of IL-8 have been associated with tumor progression and metastasis, making it a potential target for therapeutic interventions. Furthermore, the study of recombinant IL-8 protein is vital for understanding its precise biological functions and signaling mechanisms. Recombinant IL-8 can be utilized in various experimental applications, including cell migration assays, receptor binding studies, and the exploration of downstream signaling pathways. This protein is also of interest in vaccine development, as it may enhance immune responses. Given the significance of IL-8 in both health and disease, ongoing research aims to elucidate its complex role in inflammation and pathology, paving the way for potential therapeutic strategies that modulate IL-8 activity to improve patient outcomes.
