Analytical Data
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Gene name
GPR21
- Application
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Alternative Names
GPR21; Probable G-protein coupled receptor 21
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99679
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Expression Region
1-349aa
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AA Sequence
MNSTLDGNQSSHPFCLLAFGYLETVNFCLLEVLIIVFLTVLIISGNIIVIFVFHCAPLLNHHTTSYFIQTMAYADLFVGVSCVVPSLSLLHHPLPVEESLTCQIFGFVVSVLKSVSMASLACISIDRYIAITKPLTYNTLVTPWRLRLCIFLIWLYSTLVFLPSFFHWGKPGYHGDVFQWCAESWHTDSYFTLFIVMMLYAPAALIVCFTYFNIFRICQQHTKDISERQARFSSQSGETGEVQACPDKRYAMVLFRITSVFYILWLPYIIYFLLESSTGHSNRFASFLTTWLAISNSFCNCVIYSLSNSVFQRGLKRLSGAMCTSCASQTTANDPYTVRSKGPLNGCHI
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Molecular Weight
65.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GPR21, or G protein-coupled receptor 21, is a member of the GPR family of receptors and is predominantly expressed in the brain, particularly in regions associated with energy metabolism and appetite regulation. Its physiological roles have gained interest due to its potential implications in metabolic disorders, obesity, and neurodegenerative diseases. Understanding GPR21's signaling pathways and functions could provide insights into its role in the central nervous system and its interaction with various ligands. The recombinant GPR21 protein is used extensively in research to elucidate its structure-function relationships, investigate its ligand binding properties, and explore its downstream signaling mechanisms. Moreover, the generation of recombinant GPR21 allows for high-throughput screening of potential therapeutic compounds, contributing to the development of novel drugs targeted at modulating its activity for the treatment of metabolic diseases. By harnessing advanced techniques such as protein crystallization and molecular modeling, researchers aim to decipher the intricate dynamics of GPR21 and its relevance in maintaining energy homeostasis and neuronal health. The ongoing studies of GPR21 not only enhance our understanding of basic biochemistry but also hold promise for identifying new strategies in combating obesity, diabetes, and related health issues. Consequently, the recombinant expression and characterization of GPR21 remain pivotal in translating basic research into therapeutic applications, making it a valuable target for pharmaceutical development in the realm of metabolic health.
