Analytical Data
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Gene name
NSD3
- Application
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Alternative Names
NSD3；WHSC1L1；Histone-lysine N-methyltransferase NSD3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BZ95
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Expression Region
1021-1322aa
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AA Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYI DGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGA VLDIRYGVSRIAYSKDFETLK VDFLSKLPEMLKMFEDRLCHKTYLNGD HVTHPDFMLYDALDVVLYMDPMCLDAFPKLV CFKKRIEAIPQIDKYLK SSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEGDKS FAEGQT SINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKV IG KVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCP AGDRCQNQCF TKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGE LIDEEECRLRIKRAHENSVTN FYMLTVTKDRIIDAGPKGNYSRFMNHS CNPNCETQKWTVNGDVRVGLFALCDIPAGM ELTFNYNLDCLGNGRTEC HCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRK IKTEPKQMHE D
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Molecular Weight
61 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NSD3 (Nuclear receptor-binding SET domain protein 3) is a member of the NSD family of histone methyltransferases, which play critical roles in gene regulation, cell differentiation, and development. Recent studies have highlighted NSD3's significant involvement in various biological processes, including the maintenance of pluripotency in stem cells and the regulation of oncogene expression in certain cancers. Aberrant expression or mutations of NSD3 have been linked to multiple malignancies, such as acute myeloid leukemia and glioblastoma, suggesting its potential as a therapeutic target. The reconstitution of NSD3 in vitro facilitates extensive analyses of its enzymatic activity, substrate specificity, and interaction with other epigenetic modifiers, providing insights into the molecular mechanisms governing its role in gene transcription and chromatin remodeling. Understanding the functional implications of NSD3, therefore, holds promise for uncovering novel pathways in cancer biology and for the development of targeted therapies aimed at disrupting its function in tumors where it is overexpressed or mutated. As a result, NSD3 has emerged as a focal point in epigenetic research, underscoring the need for further exploration of its biological significance and the potential for intervention in NSD3-related pathologies.
