Analytical Data
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Gene name
NT5C1A
- Application
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Alternative Names
NT5C1A；Cytosolic 5'-nucleotidase 1A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BXI3
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Expression Region
1-368aa
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AA Sequence
MEPGQPREPQEPREPGPGAETAAAPVWEEAKIFYDNLAPKKKPKSPKPQN AVTIAVSSRALFRMDEEQQIYTEQGVEEYVRYQLEHENEPFSPGPAFPFV KALEAVNRRLRELYPDSEDVFDIVLMTNNHAQVGVRLINSINHYDLFIER FCMTGGNSPICYLKAYHTNLYLSADAEKVREAIDEGIAAATIFSPSRDVV VSQSQLRVAFDGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPL KGFLEALGRLQKKFYSKGLRLECPIRTYLVTARSAASSGARALKTLRSWG LETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYG VAQTPRRTAPAKQAPSAQ
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Molecular Weight
43 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NT5C1A, or cytosolic 5'-nucleotidase 1A, is an enzyme that plays a critical role in nucleotide metabolism by hydrolyzing nucleotides to produce nucleosides, which are vital for various cellular functions such as DNA and RNA synthesis. Research on NT5C1A has gained significant attention due to its implications in cancer biology, where it is associated with tumor progression and poor patient prognosis. Elevated levels of NT5C1A have been observed in several cancer types, suggesting that it may contribute to the tumor microenvironment by regulating nucleotide pools and influencing cellular signaling pathways. Furthermore, NT5C1A's ability to modulate the immune response by affecting adenosine levels has positioned it as a potential target for immunotherapy. Understanding the biochemistry of NT5C1A through recombinant protein studies can provide insights into its structure-function relationships, enzymatic properties, and regulatory mechanisms. This knowledge could pave the way for the development of novel therapeutic strategies aimed at inhibiting NT5C1A activity, thereby enhancing anti-tumor immunity and improving treatment outcomes for cancer patients. As such, NT5C1A represents a promising candidate for further investigation in both basic research and clinical applications.
