Analytical Data
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Gene name
URA5
- Application
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Alternative Names
URA5；PYR5；Orotate phosphoribosyltransferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9GZZ9
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Expression Region
1-404aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSE VVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRL FFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVEN FQHFMDRISN GGLEEGKPVD LVLSCVDNFE ARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPT TMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQE VIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLED LMAKMKNM
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Molecular Weight
47 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
URA5 is a crucial gene in yeast, particularly in the context of purine metabolism and nucleotide synthesis. It encodes a vital enzyme, orotidine 5'-phosphate decarboxylase (ODCase), which catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate, a key step in the de novo pathway of pyrimidine biosynthesis. Research on URA5 recombinant proteins has gained momentum due to their significance in understanding nucleotide biosynthesis pathways and their role in various cellular processes. The study of URA5 and its recombinant protein has implications for genetic engineering, metabolic engineering, and synthetic biology, where yeast serves as a model organism for metabolic studies. Moreover, mutations in URA5 can lead to metabolic disorders and impact yeast's growth and survival, making it an important target for research. By characterizing URA5 and its recombinant forms, researchers aim to uncover its structural and functional attributes, enhance its activity, and explore its applications in biotechnology, such as biofuel production and pharmaceutical development. Understanding URA5 and its enzymatic function provides insights into broader biological processes and can guide the development of innovative strategies for metabolic optimization in yeast and other organisms.
