Analytical Data
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基因名
PDPK1
- Application
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别名
PDPK1；PDK1；3-phosphoinositide-dependent Protein kinase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15530
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表达区间
1-556aa
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氨基酸序列
MARTTSQLYD AVPIQSSVVL CSCPSPSMVR TQTESSTPPG IPGGSRQGPA MDGTAAEPRP GAGSLQHAQP PPQPRKKRPE DFKFGKILGE GSFSTVVLAR ELATSREYAI KILEKRHIIK ENKVPYVTRE RDVMSRLDHP FFVKLYFTFQ DDEKLYFGLS YAKNGELLKY IRKIGSFDET CTRFYTAEIV SALEYLHGKG IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN SFVGTAQYVS PELLTEKSAC KSSDLWALGC IIYQLVAGLP PFRAGNEYLI FQKIIKLEYD FPEKFFPKAR DLVEKLLVLD ATKRLGCEEM EGYGPLKAHP FFESVTWENL HQQTPPKLTA YLPAMSEDDE DCYGNYDNLL SQFGCMQVSS SSSSHSLSAS DTGLPQRSGS NIEQYIHDLD SNSFELDLQF SEDEKRLLLE KQAGGNPWHQ FVENNLILKM GPVDKRKGLF ARRRQLLLTE GPHLYYVDPV NKVLKGEIPW SQELRPEAKN FKTFFVHTPN RTYYLMDPSG NAHKWCRKIQ EVWRQRYQSH PDAAVQ
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分子量
63.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDPK1, or 3-phosphoinositide-dependent protein kinase-1, is a pivotal serine/threonine kinase that plays a critical role in various cellular processes, including cell growth, metabolism, and survival. It functions at the intersection of several signaling pathways, particularly those involving the phosphoinositide 3-kinase (PI3K) pathway, which is crucial for regulating cellular responses to growth factors. Dysregulation of PDPK1 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a significant target for therapeutic interventions. The study of recombinant PDPK1 protein has gained momentum due to its potential as a biomarker and a therapeutic target. By producing this protein in a controlled laboratory setting, researchers can investigate its structure, function, and interactions with other proteins, contributing to a deeper understanding of its role in cellular signaling. Additionally, recombinant PDPK1 can serve as a valuable tool for screening small-molecule inhibitors, which may lead to new treatments for diseases associated with its dysregulation. Thus, the exploration of PDPK1 through recombinant protein research not only enhances our knowledge of fundamental cellular processes but also holds promise for the development of novel therapeutic strategies.
