Analytical Data
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Gene name
PDPK1
- Application
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Alternative Names
PDPK1；PDK1；3-phosphoinositide-dependent Protein kinase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15530
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Expression Region
1-556aa
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AA Sequence
MARTTSQLYD AVPIQSSVVL CSCPSPSMVR TQTESSTPPG IPGGSRQGPA MDGTAAEPRP GAGSLQHAQP PPQPRKKRPE DFKFGKILGE GSFSTVVLAR ELATSREYAI KILEKRHIIK ENKVPYVTRE RDVMSRLDHP FFVKLYFTFQ DDEKLYFGLS YAKNGELLKY IRKIGSFDET CTRFYTAEIV SALEYLHGKG IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN SFVGTAQYVS PELLTEKSAC KSSDLWALGC IIYQLVAGLP PFRAGNEYLI FQKIIKLEYD FPEKFFPKAR DLVEKLLVLD ATKRLGCEEM EGYGPLKAHP FFESVTWENL HQQTPPKLTA YLPAMSEDDE DCYGNYDNLL SQFGCMQVSS SSSSHSLSAS DTGLPQRSGS NIEQYIHDLD SNSFELDLQF SEDEKRLLLE KQAGGNPWHQ FVENNLILKM GPVDKRKGLF ARRRQLLLTE GPHLYYVDPV NKVLKGEIPW SQELRPEAKN FKTFFVHTPN RTYYLMDPSG NAHKWCRKIQ EVWRQRYQSH PDAAVQ
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Molecular Weight
63.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDPK1, or 3-phosphoinositide-dependent protein kinase-1, is a pivotal serine/threonine kinase that plays a critical role in various cellular processes, including cell growth, metabolism, and survival. It functions at the intersection of several signaling pathways, particularly those involving the phosphoinositide 3-kinase (PI3K) pathway, which is crucial for regulating cellular responses to growth factors. Dysregulation of PDPK1 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a significant target for therapeutic interventions. The study of recombinant PDPK1 protein has gained momentum due to its potential as a biomarker and a therapeutic target. By producing this protein in a controlled laboratory setting, researchers can investigate its structure, function, and interactions with other proteins, contributing to a deeper understanding of its role in cellular signaling. Additionally, recombinant PDPK1 can serve as a valuable tool for screening small-molecule inhibitors, which may lead to new treatments for diseases associated with its dysregulation. Thus, the exploration of PDPK1 through recombinant protein research not only enhances our knowledge of fundamental cellular processes but also holds promise for the development of novel therapeutic strategies.
