Analytical Data
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基因名
POLR1D
- Application
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别名
POLR1D；Protein POLR1D. isoform 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0DPB6
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表达区间
1-133aa
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氨基酸序列
MEEDQELERKISGLKTSMAEGERKTALEMVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRGTLPAVEPFQRGLNELMNVCQHVLDKFEASIKDYKDQKASRNESTF
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分子量
42.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR1D, a vital subunit of RNA polymerase I (Pol I), plays a crucial role in ribosomal RNA (rRNA) synthesis, which is essential for ribosome biogenesis and protein synthesis in cells. Mutations or dysfunctions in POLR1D have been associated with various human diseases, including cancer and genetic disorders like Treacher Collins syndrome. The study of POLR1D and its recombinant protein is significant for understanding the mechanisms underlying ribosome formation and cellular growth regulation. Researchers aim to explore the structural and functional properties of POLR1D to elucidate its role in rRNA transcription and how alterations in its function can lead to disease. The ability to produce recombinant POLR1D allows for more detailed biochemical analyses, including protein-protein interactions, post-translational modifications, and the effects of specific mutations. Additionally, understanding POLR1D's regulatory mechanisms could unveil novel therapeutic targets for diseases linked to ribosomal dysfunction. Overall, the investigation into POLR1D not only furthers our knowledge of fundamental biological processes but also has the potential to contribute to the development of innovative treatments for ribosomal and oncological disorders.
