Analytical Data
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Gene name
SLC16A3
- Application
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Alternative Names
SLC16A3；MCT3；MCT4；Monocarboxylate transporter 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15427
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Expression Region
1-465aa
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AA Sequence
MGGAVVDEGPTGVKAPDGGWGWAVLFGCFVITGFSYAFPKAVSVFFKELIQEFGIGYSDTAWISSILLAMLYGTGPLCSVCVNRFGCRPVMLVGGLFASLGMVAASFCRSIIQVYLTTGVITGLGLALNFQPSLIMLNRYFSKRRPMANGLAAAGSPVFLCALSPLGQLLQDRYGWRGGFLILGGLLLNCCVCAALMRPLVVTAQPGSGPPRPSRRLLDLSVFRDRGFVLYAVAASVMVLGLFVPPVFVVSYAKDLGVPDTKAAFLLTILGFIDIFARPAAGFVAGLGKVRPYSVYLFSFSMFFNGLADLAGSTAGDYGGLVVFCIFFGISYGMVGALQFEVLMAIVGTHKFSSAIGLVLLMEAVAVLVGPPSGGKLLDATHVYMYVFILAGAEVLTSSLILLLGNFFCIRKKPKEPQPEVAAAEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
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Molecular Weight
50.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC16A3, also known as Monocarboxylate Transporter 4 (MCT4), is a member of the solute carrier family and plays a crucial role in the transport of lactate and other monocarboxylates across cellular membranes. It is predominantly expressed in glycolytic tissues, such as skeletal muscle, brain, and tumor cells, where it facilitates the removal of lactate produced during anaerobic glycolysis, thereby aiding in cellular homeostasis and energy production. The dysregulation of SLC16A3 has been implicated in various pathophysiological conditions, including cancer, where it supports the acidic microenvironment and promotes tumor progression by enhancing lactate efflux. Furthermore, SLC16A3 is crucial in maintaining the metabolic adaptation of cancer cells to hypoxic conditions. The study of SLC16A3, particularly through the use of recombinant protein techniques, allows for a deeper understanding of its structural and functional properties, as well as its interactions with other cellular components. Developing recombinant proteins for SLC16A3 can provide insights into its transport mechanisms, regulatory pathways, and potential as a therapeutic target. This research holds significant implications for the development of innovative strategies in cancer treatment and metabolic disorders, highlighting the importance of SLC16A3 in both normal physiology and disease pathology.
