Analytical Data
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基因名
LPCAT4
- Application
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别名
LPCAT4；AGPAT7；AYTL3；LPEAT2；Lysophospholipid acyltransferase LPCAT4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q643R3
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表达区间
1-524aa
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氨基酸序列
MSQGSPGDWAPLDPTPGPPASPNPFVHELHLSRLQRVKFCLLGALLAPIRVLLAFIVLFLLWPFAWLQVAGLSEEQLQEPITGWRKTVCHNGVLGLSRLLFFLLGFLRIRVRGQRASRLQAPVLVAAPHSTFFDPIVLLPCDLPKVVSRAENLSVPVIGALLRFNQAILVSRHDPASRRRVVEEVRRRATSGGKWPQVLFFPEGTCSNKKALLKFKPGAFIAGVPVQPVLIRYPNSLDTTSWAWRGPGVLKVLWLTASQPCSIVDVEFLPVYHPSPEESRDPTLYANNVQRVMAQALGIPATECEFVGSLPVIVVGRLKVALEPQLWELGKVLRKAGLSAGYVDAGAEPGRSRMISQEEFARQLQLSDPQTVAGAFGYFQQDTKGLVDFRDVALALAALDGGRSLEELTRLAFELFAEEQAEGPNRLLYKDGFSTILHLLLGSPHPAATALHAELCQAGSSQGLSLCQFQNFSLHDPLYGKLFSTYLRPPHTSRGTSQTPNASSPGNPTALANGTVQAPKQKGD
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分子量
57.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LPCAT4, or lysophosphatidylcholine acyltransferase 4, is an enzyme involved in lipid metabolism, specifically in the acylation of lysophosphatidylcholine (LPC) to form phosphatidylcholine (PC), a crucial component of cell membranes. The significance of LPCAT4 has been highlighted in various studies due to its role in regulating lipid composition and influencing physiological processes such as inflammation, cell signaling, and membrane integrity. Researchers have increasingly focused on LPCAT4 in the context of metabolic disorders, cardiovascular diseases, and cancer, as alterations in phospholipid metabolism are often associated with these conditions. The recombinant expression of LPCAT4 allows for the study of its enzymatic activity, substrate specificity, and functional role in cellular contexts. Understanding the structure-function relationship of LPCAT4 can provide insights into its potential as a therapeutic target, as manipulating its activity may offer avenues for treating diseases linked to lipid dysregulation. The exploration of LPCAT4's effects on lipid profiles in various cell types further enriches our understanding of its biological significance, making it a valuable subject for ongoing research in the fields of biochemistry and molecular biology.
