Analytical Data
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Gene name
AGPAT4
- Application
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Alternative Names
(1-acylglycerol-3-phosphate O-acyltransferase 4)(1-AGP acyltransferase 4)(1-AGPAT 4)(Lysophosphatidic acid acyltransferase delta)(LPAAT-delta)
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NRZ5
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Expression Region
1-319aa
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Molecular Weight
45.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AGPAT4, or 1-Acylglycerol-3-phosphate O-acyltransferase 4, is an important enzyme involved in glycerolipid metabolism, specifically in the synthesis of triglycerides and phospholipids. Research on AGPAT4 has gained significant attention due to its role in cellular lipid homeostasis and energy storage, with implications for metabolic disorders such as obesity, diabetes, and cardiovascular diseases. Dysregulation of AGPAT4 activity can lead to alterations in lipid profiles, influencing insulin sensitivity and contributing to the development of insulin resistance. Studies have indicated that AGPAT4 may also play a role in the differentiation of adipocytes, the cells responsible for fat storage, linking it to obesity-related complications. The enzyme is localized in the endoplasmic reticulum, where it catalyzes the transfer of acyl groups to lysophosphatidic acid, forming phosphatidic acid, a key intermediate in lipid biosynthesis. Understanding AGPAT4's structure, regulation, and function could provide valuable insights into lipid metabolism and identify potential therapeutic targets for metabolic diseases. Recent advancements in recombinant protein technology and structural biology have enabled researchers to produce AGPAT4 for in-depth functional studies, facilitating the exploration of its enzymatic activity, substrate specificity, and interactions with other metabolic pathways. Additionally, the investigation of AGPAT4 polymorphisms in human populations may reveal genetic factors influencing lipid metabolism and disease susceptibility. Overall, ongoing research into AGPAT4 is crucial for unveiling the complexities of lipid metabolism and addressing the global rise in metabolic disorders.
