Analytical Data
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Gene name
virD2
- Application
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Species
grobacterium fabrum
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P18592
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Expression Region
1-447aa
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Molecular Weight
57.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VirD2 is a crucial protein involved in the process of DNA transfer within Agrobacterium tumefaciens, a bacterium that causes crown gall disease in plants. This protein plays a vital role in the T-DNA (transfer DNA) mobilization mechanism, which is essential for the bacterium's ability to transfer genetic material into the plant cells. The study of VirD2 has garnered significant interest due to its potential applications in genetic engineering and plant biotechnology. As a component of the Type IV secretion system, VirD2 is responsible for recognizing and binding to the T-DNA while facilitating its transfer. Through its interaction with other proteins in the transformation process, VirD2 serves as both a DNA processing enzyme and a marker for the T-DNA complex. Understanding the biochemical properties and mechanisms of VirD2 could lead to enhanced methods for gene delivery in plants, offering promising avenues for agricultural advancement, such as the development of disease-resistant crops or plants with improved traits. Furthermore, the manipulation of VirD2 for use in synthetic biology and the creation of novel plant transformation systems highlights the importance of ongoing research in this area. Thus, VirD2 not only serves a fundamental role in bacterial pathogenesis but also presents valuable opportunities for innovation in plant genetic modification and agricultural sustainability.
