Analytical Data
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基因名
CLTB
- Application
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别名
CLTB;Clathrin light chain B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P09497
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表达区间
1-211aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMADDFGF FSSSESGAPE AAEEDPAAAF LAQQESEIAG IENDEGFGAP AGSHAAPAQP GPTSGAGSED MGTTVNGDVF QEANGPADGY AAIAQADRLT QEPESIRKWR EEQRKRLQEL DAASKVTEQE WREKAKKDLE EWNQRQSEQV EKNKINNRAS EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR
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分子量
26 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on CLTB (Clathrin-AP180-Binding Protein) recombinant proteins is grounded in the pivotal role clathrin-mediated endocytosis plays in cellular processes. Clathrin is essential for the internalization of various substances, including hormones, nutrients, and signaling molecules, thereby facilitating communication and homeostasis within cells. CLTB, as a key adaptor protein, interacts with clathrin and other partners to orchestrate the assembly of clathrin-coated vesicles. Understanding CLTB's structure and function is crucial in elucidating the mechanisms underlying endocytosis, which has implications in several physiological and pathological contexts. Dysregulation of clathrin-mediated endocytosis is associated with diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. Researchers aim to produce recombinant CLTB proteins to investigate their biophysical properties, protein-protein interactions, and the regulatory mechanisms during vesicle formation. This foundational knowledge is expected to inform therapeutic strategies targeting endocytic pathways, offering potential interventions for diseases linked to clathrin-mediated transport dysfunction. By utilizing advanced techniques in protein engineering and molecular biology, the ongoing studies on CLTB proteins could reveal novel insights into endocytic dynamics and cellular regulation.
