Cat: PA1000-9298

Recombinant Human NOV Protein,His

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Analytical Data

  • Gene name

    NOV

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    NOV;IGFBP9;NOV;NOVH;CCN family member 3

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P48745

  • Expression Region

    28-357aa

  • AA Sequence

    M+QVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M

  • Molecular Weight

    36.2 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

NOV (Nephroblastoma Overexpressed) is a member of the CCN family of proteins, which are secreted signaling molecules involved in various cellular processes, including cell adhesion, proliferation, and differentiation. Initially identified for its overexpression in nephroblastoma, a type of kidney cancer, NOV has since attracted attention for its broader involvement in multiple biological functions and diseases. Research has shown that NOV plays a critical role in tissue development, wound healing, and fibrosis by modulating the interactions between cells and the extracellular matrix. Its dysregulation has been implicated in several pathological conditions, including cancer, cardiovascular diseases, and chronic inflammatory disorders. Due to its unique structural features, which include a cysteine-rich domain and several other functional motifs, NOV has drawn interest for its potential therapeutic applications. Understanding the mechanisms by which NOV mediates its effects on cellular behavior could lead to innovative treatment strategies for diseases characterized by aberrant cellular signaling. Furthermore, NOV's potential as a biomarker for disease progression and prognosis highlights the importance of continued research into its functional roles and regulatory pathways.

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