Analytical Data
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Gene name
CDC25A
- Application
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Alternative Names
CDC25A；M-phase inducer phosphatase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P30304
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Expression Region
1-524aa
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AA Sequence
MELGPEPPHR RRLLFACSPP PASQPVVKAL FGASAAGGLS PVTNLTVTMD QLQGLGSDYE QPLEVKNNSN LQRMGSSEST DSGFCLDSPG PLDSKENLEN PMRRIHSLPQ KLLGCSPALK RSHSDSLDHD IFQLIDPDEN KENEAFEFKK PVRPVSRGCL HSHGLQEGKD LFTQRQNSAP ARMLSSNERD SSEPGNFIPL FTPQSPVTAT LSDEDDGFVD LLDGENLKNE EETPSCMASL WTAPLVMRTT NLDNRCKLFD SPSLCSSSTR SVLKRPERSQ EESPPGSTKR RKSMSGASPK ESTNPEKAHE TLHQSLSLAS SPKGTIENIL DNDPRDLIGD FSKGYLFHTV AGKHQDLKYI SPEIMASVLN GKFANLIKEF VIIDCRYPYE YEGGHIKGAV NLHMEEEVED FLLKKPIVPT DGKRVIVVFH CEFSSERGPR MCRYVRERDR LGNEYPKLHY PELYVLKGGY KEFFMKCQSY CEPPSYRPMH HEDFKEDLKK FRTKSRTWAG EKSKREMYSR LKKL
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Molecular Weight
94 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDC25A is a dual-specificity phosphatase that plays a critical role in regulating the cell cycle, particularly in the transition from G1 to S phase. Its function involves the dephosphorylation and activation of cyclin-dependent kinases (CDKs), which are essential for cell progression and division. Dysregulation of CDC25A activity has been implicated in various cancers, as its overexpression can lead to uncontrolled cell proliferation. Research into CDC25A recombinant proteins has gained significance in the fields of cancer biology and therapeutic development, as these proteins can serve as valuable tools for understanding the molecular mechanisms of cell cycle regulation and oncogenesis. By producing and characterizing recombinant CDC25A, researchers aim to elucidate its biochemical properties, interactions with other cell cycle regulators, and potential as a therapeutic target. Furthermore, such studies can contribute to the design of inhibitors that specifically target CDC25A, offering new avenues for cancer treatment strategies and improving our overall understanding of tumor biology.
