Analytical Data
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Gene name
CYP7A1
- Application
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Alternative Names
CYP7A1；CYP17；S17AH；Steroid 17-alpha-hydroxylase/17.20 lyase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P22680
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Expression Region
1-504aa
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AA Sequence
MMTTSLIWGIAIAACCCLWLILGIRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPMDGNTTENINDTFIKTLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL
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Molecular Weight
61.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CYP7A1, or cytochrome P450 7A1, is a crucial enzyme in the cholesterol metabolism pathway, responsible for converting cholesterol into bile acids, which play a significant role in digestion and maintaining lipid homeostasis. Research on CYP7A1 has garnered significant attention due to its implications in various metabolic disorders, such as hypercholesterolemia and liver diseases. The regulation of CYP7A1 activity is influenced by numerous factors, including dietary cholesterol intake, bile acid recycling, and hormonal signaling, making it a focal point for understanding cholesterol metabolism. Reconstructing CYP7A1 as a recombinant protein allows researchers to investigate its structure-function relationships, catalytic mechanisms, and interactions with other molecules in vitro. This can aid in identifying potential therapeutic targets for cholesterol-related diseases and provide insight into the enzyme's role in drug metabolism. Additionally, studying CYP7A1 in its recombinant form allows for the exploration of genetic variants and their impact on enzyme activity, ultimately contributing to personalized medicine approaches and better management of cardiovascular health. The recombinant expression of CYP7A1 also facilitates high-throughput screening of small molecules that could modulate its activity, offering a pathway for the development of novel pharmacological agents aimed at regulating cholesterol levels and improving metabolic health outcomes.
