Analytical Data
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Gene name
dotB
- Application
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Species
Coxiella burnetii
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q83B70
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Expression Region
1-372aa
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Molecular Weight
45.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DotB is a recombinant protein that has garnered significant interest due to its potential applications in various biological and industrial fields. The protein is derived from the *Burkholderia* genus, which is known for its diverse metabolic capabilities and interactions with host organisms. DotB is primarily involved in the Type III secretion system, a sophisticated mechanism that many Gram-negative bacteria utilize to inject virulence factors into host cells, thereby facilitating infection and pathogenesis. Understanding the structure and function of DotB is crucial for elucidating the molecular pathways of bacterial invasiveness and for developing novel therapeutic strategies. Researchers have focused on characterizing DotB's role in the immune response, its interaction with host cellular mechanisms, and its potential as a target for antibiotic development. Additionally, recombinant production of DotB allows for extensive studies into its biochemical properties and functional assays, which can lead to advancements in vaccine development and diagnostics. The synthesis and purification of DotB in heterologous systems enable a more detailed investigation of its potential applications in biotechnology, including the development of biosensors and bioremediation agents. Overall, the study of DotB offers valuable insights into microbial pathogenesis and presents opportunities for innovative solutions to combat bacterial infections.
