Analytical Data
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基因名
mazE
- Application
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种属
Escherichia coli
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0AE72
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表达区间
1-82aa
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分子量
16.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The mazE protein is a key component in the study of bacterial toxin-antitoxin (TA) systems, particularly in Escherichia coli. These systems are crucial for bacterial stress responses and survival, allowing cells to enter a dormant state under adverse conditions. The mazE protein functions as an antitoxin that neutralizes the activities of the MazF toxin, which can induce cell death by cleaving mRNA and shutting down protein synthesis. Understanding the mechanisms of mazE and its interactions with MazF is vital, as it sheds light on how bacteria regulate their growth and respond to environmental stresses. Investigations into mazE have implications for developing novel antimicrobial strategies, as targeting TA systems could render bacteria more susceptible to treatment. Additionally, the study of mazE contributes to our broader knowledge of genetic regulation and cellular survival strategies in prokaryotes, making it a significant focus in microbiology and molecular biology research. Overall, the mazE protein represents an essential element in the complex interplay between toxins and antitoxins that govern bacterial life cycles and resilience.
