Analytical Data
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Gene name
SEMA3F
- Application
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Alternative Names
SEMA3F；Semaphorin-3F
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13275
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Expression Region
18-975aa
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AA Sequence
SFPTQDHLPATPRVRLSFKELKATGTAHFFNFLLNTTDYRILLKDEDHDR MYVGSKDYVLSLDLHDINREPLIIHWAASPQRIEECVLSGKDVNGECGNF VRLIQPWNRTHLYVCGTGAYNPMCTYVNRGRRAQATPWTQTQAVRGRGSR ATDGALRPMPTAPRQDYIFYLEPERLESGKGKCPYDPKLDTASALINEEL YAGVYIDFMGTDAAIFRTLGKQTAMRTDQYNSRWLNDPSFIHAELIPDSA ERNDDKLYFFFRERSAEAPQSPAVYARIGRICLNDDGGHCCLVNKWSTFL KARLVCSVPGEDGIETHFDELQDVFVQQTQDVRNPVIYAVFTSSGSVFRG SAVCVYSMADIRMVFNGPFAHKEGPNYQWMPFSGKMPYPRPGTCPGGTFT PSMKSTKDYPDEVINFMRSHPLMYQAVYPLQRRPLVVRTGAPYRLTTIAV DQVDAADGRYEVLFLGTDRGTVQKVIVLPKDDQELEELMLEEVEVFKDPA PVKTMTISSKRQQLYVASAVGVTHLSLHRCQAYGAACADCCLARDPYCAW DGQACSRYTASSKRRSRRQDVRHGNPIRQCRGFNSNANKNAVESVQYGVA GSAAFLECQPRSPQATVKWLFQRDPGDRRREIRAEDRFLRTEQGLLLRAL QLSDRGLYSCTATENNFKHVVTRVQLHVLGRDAVHAALFPPLSMSAPPPP GAGPPTPPYQELAQLLAQPEVGLIHQYCQGYWRHVPPSPREAPGAPRSPE PQDQKKPRNRRHHPPDT
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Molecular Weight
88 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SEMA3F, a member of the semaphorin family, is a class of signaling proteins known for their roles in regulating various biological processes, including neuronal development, immune responses, and tumor progression. Recent studies have highlighted the importance of SEMA3F in mediating axon guidance and cell migration, suggesting its potential as a therapeutic target in neurological disorders and cancer. Research has also shown that SEMA3F can inhibit angiogenesis and tumor growth by modulating the behavior of endothelial cells and the tumor microenvironment. The recombinant production of SEMA3F allows for detailed investigations into its functional mechanisms and therapeutic applications. It provides a platform for studying the protein's biological effects in vitro and in vivo, taking advantage of its ability to interact with specific receptors and modulate cellular signaling pathways. Understanding the structural and functional properties of SEMA3F through recombinant technology could illuminate its role in disease mechanisms and facilitate the development of novel treatment strategies in cancer therapy and neurodegenerative diseases. Overall, the study of SEMA3F and its recombinant protein holds promise for advancing our knowledge of cellular communication and developing effective interventions in various pathological conditions.
