Analytical Data
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Gene name
RNF168
- Application
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Alternative Names
(hRNF168)(RING finger protein 168)(RING-type E3 ubiquitin transferase RNF168)
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Species
Human
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Source
Baculovirus
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Tag
C- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IYW5
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Expression Region
1-571aa
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Molecular Weight
66.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF168, an E3 ubiquitin ligase, plays a crucial role in the DNA damage response (DDR) by facilitating the ubiquitination of proteins involved in DNA repair pathways. Its function is particularly important in signaling the presence of DNA double-strand breaks, where it helps recruit repair factors to the site of damage. Mutations or dysregulation of RNF168 have been associated with several human diseases, including immunodeficiencies and cancer, underscoring the need for detailed investigations into its biochemical properties and interactions. Recent studies have emphasized the significance of RNF168 in mediating the repair process through the modification of histones, leading to a better understanding of chromatin dynamics during the DDR. Research into the recombinant protein of RNF168 allows scientists to explore its enzymatic activity, interactome, and mechanism of action, paving the way for potential therapeutic strategies targeting its regulatory pathways. As such, RNF168 stands as a vital focus of study in the fields of molecular biology and cancer research, with ongoing efforts to elucidate its full spectrum of functions and implications in maintaining genomic stability.
