Analytical Data
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Gene name
walK
- Application
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Alternative Names
vicK
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Species
Staphylococcus aureus
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A6QD58
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Expression Region
382-600aa
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Molecular Weight
29.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The walK protein, part of the two-component signaling system, plays a critical role in bacterial response mechanisms, particularly in the regulation of cell wall biosynthesis and stress responses. This histidine kinase is primarily associated with the WalRK two-component system, which is crucial for maintaining cell envelope integrity in various Gram-positive bacteria. Research into walK has gained momentum due to its potential implications in understanding bacterial pathogenesis, antibiotic resistance, and cellular signaling pathways. It is known to respond to environmental cues by phosphorylating the response regulator WalR, leading to changes in gene expression that help the bacteria adapt to their surroundings. Recent studies have also highlighted walK's importance in virulence factor expression, making it a target for new antimicrobial strategies. Insights into walK function and its regulatory networks may aid the development of novel therapeutic approaches aimed at combating bacterial infections, especially in the face of rising antibiotic resistance. Overall, the study of walK represents a significant intersection of microbiology, molecular biology, and pharmaceutical research, with the potential to contribute to public health advancements.
