Analytical Data
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Gene name
mrcB
- Application
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Alternative Names
Murein polymerase pbpF, ponB
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02919
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Expression Region
444-736aa
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Molecular Weight
38.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MrcB, a key protein involved in bacterial cell division, is an essential component of the bacterial cytoskeleton. It plays a crucial role in maintaining cell shape and integrity by facilitating the proper constriction of the bacterial cell wall during division. Research on MrcB has gained increasing attention due to its potential as a target for novel antibiotics, particularly in light of the growing threat posed by antibiotic-resistant bacteria. Current studies focus on understanding the molecular mechanisms underlying MrcB function, its interaction with other cell division proteins, and its overall contribution to bacterial physiology. The recombinant production of MrcB protein not only allows researchers to study its biochemical properties in detail but also aids in the development of high-throughput screening assays for drug discovery. Furthermore, MrcB's structural features, such as its oligomeric state and conformational changes during the cell division process, are critical areas of investigation. Advances in this field could pave the way for innovative therapeutic approaches to combat bacterial infections, making MrcB a focal point in microbiological and pharmaceutical research.
