Analytical Data
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Gene name
ccmK2
- Application
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Species
Synechocystis sp.
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P72761
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Expression Region
2-103aa
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Molecular Weight
18.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of ccmK2 recombinant protein is rooted in the exploration of its role in the biogenesis of cytochrome c, a vital component involved in electron transport chains across various organisms. Cytochrome c is essential for cellular respiration and energy production, making it a key focus in bioenergetics and mitochondrial studies. The ccm locus, particularly ccmK2, is known to be involved in the maturation and membrane integration of cytochrome c in bacteria, particularly in the model organism Escherichia coli. Research aimed at understanding the structure and function of ccmK2 is crucial as it can reveal insights into how cytochrome c is synthesized and processed within cells. Recombinant expression of ccmK2 allows for the production of the protein in a controlled environment, facilitating detailed biochemical characterization. These studies may elucidate the protein’s role in the interaction with heme and its implications in various pathogenic bacteria, where cytochrome c can influence virulence and metabolic pathways. Additionally, investigating ccmK2 contributes to broader studies in synthetic biology and biotechnological applications, such as developing microbial platforms for bioenergy production and bioremediation. Overall, the research on ccmK2 recombinant protein not only advances our understanding of fundamental biological processes but also holds promise for practical applications in health and environmental sustainability.
