Analytical Data
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Gene name
vvhA
- Application
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Alternative Names
vvhA; VV2_0404; Cytolysin
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Species
Vibrio vulnificus
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19247
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Expression Region
21-471aa
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Molecular Weight
55.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of vvhA recombinant protein is situated within the broader context of understanding the pathogenic mechanisms of Vibrio splendidus, a marine bacterium known to cause severe diseases in various aquatic organisms, including economically important fish and shellfish. The vvhA gene encodes for a hemolysin, a type of exotoxin that plays a critical role in the bacterium's virulence. By investigating the structure and function of the vvhA recombinant protein, researchers aim to elucidate its role in host-pathogen interactions and the resulting cellular damage. This research is essential not only for comprehending the molecular basis of Vibrio infections but also for developing effective therapeutic strategies and vaccines to mitigate the impact of this pathogen on aquaculture. Furthermore, studies focusing on vvhA may contribute to the understanding of similar virulence factors in other bacterial species, potentially leading to broader applications in microbial pathogenesis research. The production of vvhA recombinant protein allows for detailed analyses such as functional assays, binding studies, and immunogenicity tests, thereby facilitating advancements in both fundamental and applied microbiology.
