Analytical Data
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Gene name
ihfB
- Application
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Alternative Names
/
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Species
Neisseria meningitidis serogroup B
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A0U2
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Expression Region
1-104aa
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Molecular Weight
17.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The IHF (Integration Host Factor) is a crucial architectural protein found in bacteria, playing a significant role in DNA packaging, transcription regulation, and the facilitation of DNA replication. Among the various types of IHF, the ihfB gene encodes one of its subunits, which is essential for the protein's function in maintaining DNA structure and stability. Research on recombinant IHF proteins, particularly ihfB, has gained momentum due to their potential applications in synthetic biology and biotechnology. By producing ihfB in a recombinant system, researchers can investigate its structural properties and interactions with DNA, providing insights into its function within cellular processes. Additionally, understanding the molecular mechanisms of IHF can lead to advancements in genetic engineering techniques, including CRISPR-Cas systems and targeted gene editing. Furthermore, studying recombinant ihfB can contribute to the development of novel antimicrobial strategies, as manipulating IHF activity may disrupt bacterial growth and survival. Thus, the exploration of ihfB recombinant protein opens avenues for both fundamental microbiology research and practical applications in medicine and biotechnology, highlighting its importance in the scientific community.
