Analytical Data
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Gene name
ARHGEF12
- Application
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Alternative Names
Leukemia-associated RhoGEF
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NZN5
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Expression Region
367-558aa
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Molecular Weight
28.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ARHGEF12, also known as Trio, is a member of the Rho guanine nucleotide exchange factor (GEF) family, which plays a critical role in modulating Rho GTPase signaling pathways. These pathways are essential for various cellular processes, including cell migration, adhesion, and cytoskeletal dynamics, which are pivotal during developmental processes and in maintaining tissue homeostasis. Dysregulation of ARHGEF12 has been implicated in several pathological conditions, including cancer and cardiovascular diseases, making it a significant target for therapeutic interventions. Research into ARHGEF12 often focuses on its structure-function relationships, the mechanisms through which it activates Rho GTPases, and its interactions with other signaling molecules. Recombinant ARHGEF12 protein is utilized in these studies to elucidate its role in cellular signaling and to explore its potential as a biomarker or therapeutic target. The production of this protein in a laboratory setting allows for detailed in vitro assays, providing insights into how ARHGEF12 contributes to disease progression and identifying possible avenues for drug development. Understanding the functional characteristics of ARHGEF12 not only enhances our knowledge of cellular signaling networks but also holds promise for innovative treatment strategies aimed at diseases associated with its dysfunction.
