Analytical Data
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Gene name
type
- Application
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Alternative Names
B2 metallo-beta-lactamase BLA-IMP Beta-lactamase type II Metallo-beta-lactamase type II
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Species
Serratia marcescens
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52699
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Expression Region
19-246aa
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Molecular Weight
32.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Type重组蛋白(recombinant protein)研究在生物技术和生物医药领域中占据了重要地位。这种蛋白质是通过基因工程技术在宿主细胞中合成的,通常是利用大肠杆菌、酵母细胞、昆虫细胞或哺乳动物细胞作为表达系统。随着基因组学和蛋白质组学的发展,研究者可以方便地克隆编码特定蛋白的基因,从而高效生产具有特定结构和功能的重组蛋白。重组蛋白广泛应用于疫苗开发、抗体生成、酶工程以及生物标志物的研究等领域。尤其是在疫苗开发中,重组蛋白疫苗因其安全性和有效性备受关注。同时,这类蛋白也是新药研发中关键的生物制剂,有助于治疗多种疾病,包括癌症和自身免疫病。尽管重组蛋白技术已经取得显著进展,研究者仍面临着提高蛋白质产量、正确折叠和后翻译修饰等挑战。因此,深入研究与优化重组蛋白的生产和纯化过程,将进一步推动其在临床和工业应用中的潜力。











