Analytical Data
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基因名
NDUFA3
- Application
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别名
Complex I-B9 ;CI-B9NADH-ubiquinone oxidoreductase B9 subunit
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种属
Human
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95167
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表达区间
2-84aa
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分子量
36.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFA3, a critical subunit of the mitochondrial respiratory chain complex I, plays a vital role in cellular energy metabolism. This protein is essential for the assembly and stability of complex I, which is responsible for the critical task of oxidative phosphorylation in eukaryotic cells. Dysfunction or mutations in NDUFA3 have been implicated in various mitochondrial diseases and disorders, including neurodegenerative conditions and metabolic syndromes. Research on NDUFA3 recombinant protein has gained attention as it allows for the exploration of its structural and functional properties, facilitating our understanding of the biochemical pathways involved in ATP production. By producing NDUFA3 in a recombinant form, scientists can investigate the effects of specific mutations, delineate the protein's interactions within the electron transport chain, and evaluate potential therapeutic interventions for associated mitochondrial dysfunctions. Furthermore, studies of NDUFA3 could provide insights into broader mitochondrial biology, including signaling pathways related to apoptosis, oxidative stress, and cellular response to energy demands. Understanding NDUFA3 at the molecular level not only sheds light on fundamental biological processes but also holds promise for developing strategies to combat diseases associated with mitochondrial dysfunctions. Hence, research focusing on the recombinant expression and characterization of NDUFA3 is crucial for advancing our comprehension of mitochondrial biology and its implications for human health.












