Analytical Data
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基因名
FGF-12
- Application
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生物活性
1. The ED50 is <20>5 × 104 units/mg. 2. Immobilized Human FGF-12 at 2.0 μg/ml (100 μl/well) can bind Human FGFR3-Fc. The ED50 of Human FGF-12 is 0.5-4.0 μg/ml. 3. Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED50 this effect is 9.139 ng/mL, corresponding to a specific activity is 1.094×10^5 U/mg. easured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 9.139 ng/mL, corresponding to a specific activity is 1.094×105 U/mg.
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别名
rHuFGF-12; FHF1; FGF12B
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P61328-1
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表达区间
E67-T243
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氨基酸序列
MESKEPQLKGIVTRLFSQQGYFLQMHPDGTIDGTKDENSDYTLFNLIPVGLRVVAIQGVKASLYVAMNGEGYLYSSDVFTPECKFKESVFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEVCMYREPSLHEIGEKQGRSRKSSGTPTMNGGKVVNQDST
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蛋白长度
Partial
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分子量
21 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fibroblast growth factor 12 (FGF-12) is a member of the FGF family, which plays a crucial role in various biological processes, including cell proliferation, differentiation, and survival. Unlike other FGFs, FGF-12 does not exhibit classical growth factor functions, as it lacks the capability to bind to FGF receptors effectively. Instead, FGF-12 is predominantly expressed in the central nervous system and has been implicated in modulating neuronal excitability and synaptic transmission. Its unique properties suggest potential roles in neurodevelopmental disorders and neurodegenerative diseases. Research into recombinant FGF-12 proteins has gained traction due to their potential applications in understanding the underlying mechanisms of neurological functions and pathologies. Recombinant FGF-12 is utilized to investigate its interactions with intracellular signaling pathways and to explore its therapeutic potential in restoring normal neuronal function. Studies have shown that FGF-12 may influence the activity of ion channels, thereby affecting neuronal excitability and signaling. Furthermore, understanding the structural characteristics of FGF-12 through recombinant technology can provide insights into the functional diversity of FGFs in the nervous system. As researchers delve deeper into the functional aspects of FGF-12, it may pave the way for novel therapeutic strategies targeting various neurological conditions, emphasizing the importance of this protein in the field of neurobiology.
