Analytical Data
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基因名
CPSF
- Application
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别名
Cleavage and polyadenylation specificity factor 30KDA subunit
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种属
Human
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95639
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表达区间
1-244aa
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分子量
54.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CPSF (Cleavage and Polyadenylation Specificity Factor) is a multi-protein complex that plays a crucial role in the process of pre-mRNA processing, particularly in the cleavage and polyadenylation of the 3' end of mRNA transcripts. The correct cleavage and subsequent addition of the poly(A) tail are essential for mRNA stability, nuclear export, and translation, making CPSF a significant factor in gene expression regulation. Research into CPSF has revealed its involvement in various cellular processes, including the response to cellular stress and the regulation of mRNA half-life. Understanding the structure and function of CPSF is critical, as dysregulation of polyadenylation and pre-mRNA processing can lead to various diseases, including cancer and neurological disorders. Recent advances in recombinant protein technology have enabled the production of purified CPSF components, facilitating detailed biochemical characterization and structural studies. These studies have provided valuable insights into the interactions between CPSF and other RNA processing factors, helping to elucidate the regulatory mechanisms underlying gene expression. Given the essential role of CPSF in mRNA metabolism, ongoing research aims to further explore its functional dynamics and potential as a therapeutic target in disease contexts.
