Analytical Data
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Gene name
ASMT
- Application
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Alternative Names
Hydroxyindole O-methyltransferase ( HIOMT)
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46597
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Expression Region
1-345aa
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Molecular Weight
45.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ASMT (Acetylserotonin O-methyltransferase) is an important enzyme involved in the biosynthesis of melatonin, a hormone that regulates circadian rhythms and sleep-wake cycles. The study of ASMT recombinant proteins has gained attention due to the enzyme's critical role in melatonin production and its implications for sleep disorders, seasonal affective disorder, and other health conditions. Historically, the understanding of ASMT's structure and function has been limited, primarily due to difficulties in obtaining large amounts of functional protein from natural sources. Advances in molecular biology techniques now allow for the expression of ASMT as a recombinant protein, facilitating detailed studies on its enzymatic activity, substrate specificity, and interaction with other molecules involved in melatonin synthesis. By producing ASMT in model organisms or cell systems, researchers can explore the enzyme’s regulation, post-translational modifications, and its role in various physiological processes. Furthermore, recombinant ASMT can be utilized in pharmacological studies and therapeutic applications, such as developing new treatments for sleep-related disorders or exploring melatonin's broader effects on health. Understanding the intricacies of ASMT through recombinant protein studies can lead to significant advancements in both basic science and clinical practices, making it a focal point of research in biochemistry and neurobiology.
