Analytical Data
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Gene name
HLA-A*02:01&B2M&HPV16 E7(YMLDLQPET) Tetramer
- Application
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Alternative Names
rHuHLA-A*0201 HPV16 E7 complex Protein
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P04439-1 (G25-T305)&P61769
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Expression Region
P04439-1 (G25-T305)&P61769 (I21-M119)&YMLDLQPET
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Molecular Weight
260-265 kDa under Non reducing (N)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HLA-A*02:01 is a well-characterized major histocompatibility complex (MHC) class I molecule that presents peptide antigens to CD8+ T cells, playing a crucial role in immune responses against viral infections and tumor cells. The E7 protein of human papillomavirus type 16 (HPV16), a key oncoprotein associated with cervical cancer, contains the peptide YMLDLQPET, which is recognized by T cells in individuals expressing the HLA-A*02:01 allele. The study of HLA-A*02:01/B2M/HPV16 E7 (YMLDLQPET) tetramer recombinant protein enables the identification and isolation of specific T cells that recognize this antigen. This approach is vital for understanding the immune response against HPV-associated tumors and has potential applications in immunotherapy and vaccine development. By using tetramers, researchers can quantitatively analyze T cell populations and their functionality, contributing to advancements in personalized medicine for HPV-related malignancies. This research highlights the importance of targeting specific MHC-peptide complexes to enhance anti-tumor immunity and improve therapeutic strategies for HPV-associated diseases.
