Analytical Data
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Gene name
6Ckine/CCL21A
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简介
6Ckine/CCL21B Protein, Mouse is a homeostatic lymphoid chemokine that contributes to the entry of T cells and dendritic cells into the lymphoid T-zone. It acts through chemokine receptors CCR7 and CXCR3 to promote fibrogenic and inflammatory cytokine production. Exodus-2/CCL21 Protein, Mouse is a recombinant mouse 6Ckine/CCL21B (S24-G133) expressed by E. coli[1].
- Application
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Biological Activity
1.Full biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/mL. 2.Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.The ED50 for this effect is 13.57 ng/mL, corresponding to a specific activity is 7.37×104 U/mg. Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7 .The ED50 for this effect is 13.57 ng/mL, corresponding to a specific activity is 7.37×104 U/mg.
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Alternative Names
6Ckine; CCL21B; Beta-chemokine exodus-2;
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P86792
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Expression Region
S24-G133
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Protein Length
Full Length of Mature Protein
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Molecular Weight
17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The chemokine 6Ckine, also known as CCL21A, plays a crucial role in the immune system by mediating the migration and activation of lymphocytes, particularly in the context of immune responses and inflammation. As a member of the CC chemokine family, CCL21A is primarily produced by lymphoid tissues and is involved in the retention and circulation of immune cells within lymph nodes. Its ability to bind to the CCR7 receptor on T cells and dendritic cells is essential for orchestrating adaptive immune responses, making it a significant focus of research in immunology. Recent studies have highlighted the potential therapeutic applications of CCL21A in cancer immunotherapy and autoimmune diseases, where enhancing lymphocyte trafficking could improve treatment outcomes. The recombinant production of 6Ckine/CCL21A allows for detailed investigation of its biological functions and facilitates the development of novel immunotherapeutic strategies by providing a valuable tool for studying its interactions with receptors and signaling pathways. Understanding the role of CCL21A in immune regulation could lead to new insights into disease mechanisms and innovative treatment approaches.
