Analytical Data
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Gene name
DKK-1 N terminal Domain
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简介
DKK-1 Proteinas are potent antagonists of canonical Wnt signaling, inhibiting LRP5/6-Wnt interactions and forming a ternary complex with KREMEN to promote LRP5/6 internalization.In addition to its proapoptotic function by antagonizing KREMEN1 independently of Wnt, DKK-1 also exhibits antiapoptotic activity.DKK-1 N terminal Domain Protein, Human (HEK293, Fc-Avi) is the recombinant human-derived DKK-1 N terminal Domain protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.
- Application
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Alternative Names
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O94907
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Expression Region
T32-D142
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Protein Length
Partial
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Molecular Weight
50-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The DKK-1 (Dickkopf-related protein 1) N-terminal domain is a crucial component of the canonical Wnt signaling pathway, which plays a significant role in various biological processes such as embryonic development, cell differentiation, and tissue homeostasis. DKK-1 functions as an antagonist of Wnt signaling by binding to LRP5/LRP6 co-receptors, thereby inhibiting Wnt-induced signaling cascades. Research into the reconstitution of the DKK-1 N-terminal domain as a recombinant protein is driven by its potential applications in therapeutic interventions for diseases where Wnt signaling is dysregulated, including cancers, osteoporosis, and other bone disorders. Moreover, understanding the structure and function of the DKK-1 N-terminal domain provides insight into its interactions with other molecular partners and its role in cellular signaling. Studies utilizing recombinant DKK-1 protein can further elucidate its mechanistic functions, paving the way for the development of targeted therapies that manipulate Wnt signaling pathways. Insights gained from such research may contribute to novel strategies for treating malignancies and metabolic bone diseases, highlighting the importance of DKK-1 in both basic and translational research.
