Analytical Data
-
基因名
Collagen alpha-1(IX) chain/COL9A1
-
简介
Collagen alpha-1(IX) chain (COL9A1) is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage and a structural component of vitreous in the eye. Synthesis of COL9A1 chain is essential for assembly of type IX collagen molecules, and mutations of COL9A1 are associated with osteoarthritis in humans, multiple epiphyseal dysplasia, and Stickler syndrome. Collagen alpha-1 (IX) chain/COL9A1 Protein, Human (HEK293, His) is the recombinant human-derived Collagen alpha-1(IX) chain/COL9A1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
-
别名
rHuCollagen alpha-1(IX) chain/COL9A1, His; Collagen alpha-1(IX) chain; DJ149L1.1.2; EDM6; MED; STL4
-
种属
Human
-
表达系统
HEK293
-
标签
C-6*His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P20849-3
-
表达区间
A24-G598
-
氨基酸序列
AVKRRPRFPVNSNSNGGNELCPKIRIGQDDLPGFDLISQFQVDKAASRRAIQRVVGSATLQVAYKLGNNVDFRIPTRNLYPSGLPEEYSFLTTFRMTGSTLKKNWNIWQIQDSSGKEQVGIKINGQTQSVVFSYKGLDGSLQTAAFSNLSSLFDSQWHKIMIGVERSSATLFVDCNRIESLPIKPRGPIDIDGFAVLGKLADNPQVSVPFELQWMLIHCDPLRPRRETCHELPARITPSQTTDERGPPGEQGPPGPPGPPGVPGIDGIDGDRGPKGPPGPPGPAGEPGKPGAPGKPGTPGADTSP
-
蛋白长度
Partial
-
分子量
38-50 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Collagen alpha-1(IX) chain, or COL9A1, is a vital component of the extracellular matrix, primarily found in cartilage and intervertebral discs. This protein plays a crucial role in maintaining the structural integrity and function of these tissues, contributing to the mechanical properties essential for load-bearing and support. Abnormalities in COL9A1 expression or function are linked to various skeletal disorders, such as osteoarthritis and intervertebral disc degeneration, highlighting its significance in both developmental and pathological contexts. The study of COL9A1 recombinant protein has gained attention due to its potential applications in regenerative medicine and tissue engineering. By producing and characterizing this protein, researchers aim to gain insights into its structural properties and interactions with other matrix components. Furthermore, understanding the post-translational modifications and functional mechanisms of COL9A1 may aid in developing therapeutic strategies for conditions associated with cartilage and disc degeneration. The creation of recombinant COL9A1 not only provides a model for studying its biological roles but also offers opportunities for drug delivery systems and biomaterials that can mimic the natural extracellular matrix environment. In summary, COL9A1 is significant for its contributions to cartilage health, and its recombinant form serves as a valuable tool for advancing research in both basic biology and clinical applications.
