Analytical Data
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Gene name
DKK-1
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简介
DKK-1 Proteinas are potent antagonists of canonical Wnt signaling, inhibiting LRP5/6-Wnt interactions and forming a ternary complex with KREMEN to promote LRP5/6 internalization. In addition to its proapoptotic function by antagonizing KREMEN1 independently of Wnt, DKK-1 also exhibits antiapoptotic activity. DKK-1 Protein, Rhesus Macaque (N256Q, HEK293, His) is the recombinant Rhesus Macaque-derived DKK-1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
Dickkopf-related protein 1; Dickkopf-1; Dkk-1; SK; DKK1
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
XP_001098844.1
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Expression Region
T32-H266, N256Q
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Protein Length
Partial
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Molecular Weight
38-43 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DKK-1 (Dickkopf-1) is a secreted glycoprotein that plays a crucial role in developmental biology and cellular signaling, particularly in the regulation of the Wnt signaling pathway. Initially discovered for its involvement in embryonic development, DKK-1 has garnered significant attention due to its dual role as a regulator of bone metabolism and a potential biomarker in various diseases, including cancer and osteoporosis. Elevated levels of DKK-1 have been associated with the inhibition of osteoblast differentiation and activity, thereby contributing to bone mass reduction. Moreover, its expression is upregulated in certain malignancies, suggesting a role in tumor progression and immune evasion. As a result, researchers have focused on the potential therapeutic implications of DKK-1 modulation, exploring the use of DKK-1 antagonists or neutralizing antibodies to enhance bone formation and improve cancer treatment outcomes. Recent studies have also delved into the recombinant production of DKK-1 for experimental and therapeutic applications, enabling clearer insights into its biological functions and effects on various pathological conditions. This research aims to unravel the complex mechanisms of DKK-1 in tissue homeostasis and disease, revealing new avenues for therapeutic intervention.
