Analytical Data
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基因名
NAGA
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简介
NAGA proteins play a crucial role in cellular metabolism, catalyzing the removal of terminal α-N-acetylgalactosamine residues from glycolipids and glycopeptides. This enzyme activity is essential for the efficient breakdown of glycolipids, contributing to the overall catabolic process within the cell. NAGA Protein, Human (HEK293, His) is the recombinant human-derived NAGA protein, expressed by HEK293 , with C-His labeled tag.
- Application
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别名
Alpha-N-acetylgalactosaminidase; Alpha-galactosidase B; NAGA
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P17050
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表达区间
L18-Q411
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氨基酸序列
LDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDDIQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPLLMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNFTGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQ
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蛋白长度
Full Length of Mature Protein
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分子量
47-65 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NAGA, or N-acetylgalactosamine-6-sulfatase, is an essential enzyme involved in the lysosomal degradation of glycosaminoglycans, specifically heparan sulfate and dermatan sulfate. Deficiencies in NAGA are linked to a rare genetic disorder known as Morquio A syndrome, which results in skeletal abnormalities, organ dysfunction, and reduced mobility. Research on NAGA recombinant proteins aims to provide insights into the enzyme's structure and function, as well as potential therapeutic interventions for affected individuals. By producing NAGA in a recombinant system, researchers can overcome limitations associated with natural enzyme extraction, such as low yield and potential contamination. These recombinant proteins can also serve as valuable tools for studying the enzyme's activity, substrate specificity, and interaction with other lysosomal enzymes. Furthermore, the development of recombinant NAGA may pave the way for enzyme replacement therapies, offering hope for patients with Morquio A syndrome by restoring the missing enzyme activity and improving the overall pathology associated with the disorder. This line of research not only contributes to understanding lysosomal storage disorders but also opens avenues for developing innovative treatments that enhance patients' quality of life.
