Analytical Data
-
Gene name
PIKFYVE
- Application
-
Alternative Names
PIP5K3; CFD; PIP5K; PIKfyve; p235; Phosphatidylinositol-3-Phosphate/Phosphatidylinositol 5-Kinase,Type III; 1-phosphatidylinositol 3-phosphate 5-kinase
-
Species
Mouse
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9Z1T6
-
Expression Region
Thr1725~Glu1976
-
Molecular Weight
33kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIKFYVE, a phosphoinositide-5-kinase, is an essential enzyme that plays a pivotal role in lipid signaling and membrane dynamics. Research on PIKFYVE has gained momentum due to its implications in various cellular processes, including endocytosis, autophagy, and neuronal signaling. It is particularly noteworthy for its involvement in the synthesis of phosphoinositides, which are critical for cellular signaling pathways and membrane trafficking. Dysregulation of PIKFYVE has been linked to several diseases, such as cancer and neurodegenerative disorders, making it a potential target for therapeutic intervention. The study of recombinant PIKFYVE has become increasingly important for dissecting its biochemical properties, identifying binding partners, and understanding its functional mechanisms in cellular contexts. By producing PIKFYVE as a recombinant protein, researchers can perform in vitro assays to study its enzymatic activity and interactions with various substrates and regulators. This research not only aids in elucidating the physiological roles of PIKFYVE but also helps in the development of inhibitors that could serve as novel therapeutic agents. The ongoing investigation around PIKFYVE underscores its significance in cell biology and its potential as a drug target, paving the way for advancements in understanding and treating diseases associated with its dysfunction.
