Analytical Data
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Gene name
Semaphorin-4B/SEMA4B
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简介
Semaphorin-4B (SEMA4B) Protein, functioning as an inhibitor, crucially modulates axonal extension by providing localized signals that specify territories as inaccessible for growing axons. Semaphorin-4B/SEMA4B Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4B/SEMA4B protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
Semaphorin-4B; KIAA1745; SEMAC and SEMA4B
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPR2-1
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Expression Region
L44-E717
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AA Sequence
LSPRISLPLGSEERPFLRFEAEHISNYTALLLSRDGRTLYVGAREALFALSSNLSFLPGGEYQELLWGADAEKKQQCSFKGKDPQRDCQNYIKILLPLSGSHLFTCGTAAFSPMCTYINMENFTLARDEKGNVLLEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSLRPTKTESSLNWLQDPAFVASAYIPESLGSLQGDDDKIYFFFSETGQEFEFFENTIVSRIARICKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLSPSPQDWRDTLFYGVFTSQWHRGTTEGSAVCVFTMKDVQRVFSGLYKEVNRETQQWYTVTHPVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRMLLLQPQARYQRVAVHRVPGLHHTYDVLFLGTGDGRLHKAVSVGPRVHIIEELQIFSSGQPVQNLLLDTHRGLLYAASHSGVVQVPMANCSLYRSCGDCLLARDPYCAWSGSSCKHVSLYQPQLATRPWIQDIEGASAKDLCSASSVVSPSFVPTGEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAPVNASASCHVLPTGDLLLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSVPVIISTSRVSAPAGGKASWGADRSYWKE
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Protein Length
Extracellular Domain
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Molecular Weight
86 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Semaphorin-4B (SEMA4B) is a member of the semaphorin family, which plays a crucial role in neuronal development and axon guidance, as well as in the regulation of immune responses and cancer progression. Research has shown that SEMA4B interacts with various receptors, such as Plexin-B1 and Neuropilin-1, influencing cellular signaling pathways that are pivotal for cell migration, differentiation, and apoptosis. Notably, SEMA4B is implicated in several pathological conditions, including autoimmune diseases and malignancies, making it a target of interest for therapeutic interventions. The creation of recombinant SEMA4B proteins has enabled researchers to study its functional effects in vitro and in vivo, providing insights into its biological mechanisms and potential as a biomarker or therapeutic agent. Investigating the structure-function relationship of SEMA4B through recombinant protein studies can further elucidate its role in the tumor microenvironment and immune modulation, highlighting its relevance in both neuroscience and immunology. Overall, the exploration of SEMA4B offers promising avenues for understanding complex biological interactions and developing novel strategies for disease management.
