Analytical Data
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Gene name
WFS1
- Application
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Alternative Names
DFNA14; DFNA38; DFNA6; DIDMOAD; WFRS; WFS; Wolframin
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O76024
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Expression Region
Met1~Lys300
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Molecular Weight
37kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
WFS1, or Wolframin, is a protein encoded by the WFS1 gene, primarily known for its role in Wolfram syndrome, a rare genetic disorder characterized by diabetes, optic atrophy, and deafness. Research into WFS1 has gained significant attention due to its diverse functions in cellular processes, including ion homeostasis and stress response. The protein is localized in the endoplasmic reticulum (ER) and is believed to play a critical role in maintaining ER integrity and calcium signaling. Mutations in the WFS1 gene disrupt these functions, leading to the pathophysiological manifestations of Wolfram syndrome. Recent studies have highlighted its involvement in mitochondrial function and neuronal survival, suggesting a broader role in neurodegenerative diseases and metabolic disorders. Understanding the structure and function of WFS1 through recombinant protein studies is essential for uncovering the mechanisms behind its pathogenicity. This research not only sheds light on Wolfram syndrome but also opens avenues for therapeutic interventions targeting WFS1-related pathways in various diseases. As scientists delve deeper into the molecular mechanisms of WFS1, recombinant protein technology serves as a vital tool for elucidating its functional dynamics, making it a focal point in both basic and translational research.
