Analytical Data
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Gene name
H2AFY
- Application
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Alternative Names
H2A.y; H2A/y; H2AF12M; mH2A1; macroH2A1.2; Core histone macro-H2A.1; Medulloblastoma antigen MU-MB-50.205
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Species
Human
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Source
E. coli
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Tag
N- His & GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75367
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Expression Region
Ile109~Asn369
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Molecular Weight
57/39kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
H2AFY, also known as H2A.Z, is a variant of the histone H2A protein, which plays a crucial role in the regulation of chromatin structure and function. Histone variants like H2AFY are essential for various cellular processes, including gene expression, DNA repair, and replication. The unique properties of H2AFY, such as its ability to replace conventional H2A in nucleosomes, contribute to the formation of specialized chromatin domains that influence transcriptional regulation. Research into H2AFY has gained momentum in recent years due to its involvement in developmental processes and disease states, particularly in cancer. Abnormalities in H2AFY expression or function can lead to altered chromatin dynamics and gene regulation, resulting in tumorigenesis. Understanding the structure and function of H2AFY, as well as its interactions with other chromatin-associated proteins, is critical for elucidating its role in health and disease. Recombinant H2AFY protein production allows researchers to investigate its biochemical properties, study its interactions with DNA and other histones, and assess its impact on chromatin configuration. This research has the potential to unveil novel mechanisms of gene regulation and provide insights into therapeutic targets for diseases where H2AFY is implicated. Overall, the study of H2AFY and its recombinant forms represents a significant frontier in chromatin biology, with implications for understanding genetic regulation and developing innovative strategies for disease intervention.
