Analytical Data
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基因名
BCKDK
- Application
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别名
BCKDHKIN; 3-Methyl-2-Oxobutanoate Dehydrogenase/Lipoamide Kinase,Mitochondrial
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种属
Rat
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q00972
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表达区间
Arg44~Thr223
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分子量
24kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BCKDK, or Branched-Chain Keto Acid Dehydrogenase Kinase, plays a crucial role in the regulation of branched-chain amino acid (BCAA) metabolism, which is vital for energy production, protein synthesis, and maintaining metabolic homeostasis. The interest in BCKDK has surged due to its associations with various metabolic disorders, including obesity, diabetes, and certain types of cancer. Dysregulation of BCAA metabolism can lead to elevated levels of these amino acids and their corresponding keto acids, which have been linked to insulin resistance and other metabolic syndromes. Furthermore, recent studies have indicated that BCKDK may influence cell growth and proliferation, making it a potential target for therapeutic interventions. Researchers are exploring the functional mechanisms of BCKDK and its impact on cellular metabolism, signaling pathways, and overall energy balance. By understanding the molecular details of BCKDK's action, scientists aim to develop novel strategies to modulate its activity, offering insights into treatment options for metabolic diseases and cancer. Thus, the investigation of BCKDK and its recombinant proteins is a promising area of research that holds the potential to unveil new approaches to address pressing health issues related to metabolic dysfunction.
