Analytical Data
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Gene name
BCKDK
- Application
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Alternative Names
BCKDHKIN; 3-Methyl-2-Oxobutanoate Dehydrogenase/Lipoamide Kinase,Mitochondrial
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Species
Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q00972
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Expression Region
Arg44~Thr223
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Molecular Weight
24kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BCKDK, or Branched-Chain Keto Acid Dehydrogenase Kinase, plays a crucial role in the regulation of branched-chain amino acid (BCAA) metabolism, which is vital for energy production, protein synthesis, and maintaining metabolic homeostasis. The interest in BCKDK has surged due to its associations with various metabolic disorders, including obesity, diabetes, and certain types of cancer. Dysregulation of BCAA metabolism can lead to elevated levels of these amino acids and their corresponding keto acids, which have been linked to insulin resistance and other metabolic syndromes. Furthermore, recent studies have indicated that BCKDK may influence cell growth and proliferation, making it a potential target for therapeutic interventions. Researchers are exploring the functional mechanisms of BCKDK and its impact on cellular metabolism, signaling pathways, and overall energy balance. By understanding the molecular details of BCKDK's action, scientists aim to develop novel strategies to modulate its activity, offering insights into treatment options for metabolic diseases and cancer. Thus, the investigation of BCKDK and its recombinant proteins is a promising area of research that holds the potential to unveil new approaches to address pressing health issues related to metabolic dysfunction.
