Analytical Data
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Gene name
EEA1
- Application
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Alternative Names
ZFYVE2; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15075
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Expression Region
His1232~Lys1348
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Molecular Weight
17kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EEA1 (Early Endosome Antigen 1) is a critical protein involved in the early stages of endocytosis and the trafficking of membrane and cytoplasmic components within eukaryotic cells. Initially identified as an antigenic marker for early endosomes, EEA1 plays a pivotal role in the maturation of these organelles and serves as a key regulator in the fusion processes of endosomes, helping to ensure proper cellular recycling and degradation pathways. The protein contains a FYVE domain that facilitates its binding to phosphatidylinositol 3-phosphate (PI3P)-rich membranes, a crucial step for its localization to early endosomes. Research in recent years has expanded our understanding of EEA1's multifaceted roles beyond endosomal dynamics, including its involvement in various cellular functions such as signal transduction, stress responses, and viral entry mechanisms. Given the implications of EEA1 in cellular homeostasis and its link to diseases such as cancer and neurodegenerative disorders, the study of its recombinant forms has garnered significant interest. Such recombinant EEA1 proteins allow for in-depth investigations into their structural, functional, and interaction properties, facilitating the exploration of how mutations or alterations in EEA1 activity can impact cellular processes. By using recombinant proteins, researchers aim to unravel the complexities of EEA1's role in endosomal trafficking and its broader implications in health and disease, thus providing insights that can inform therapeutic strategies targeting abnormalities associated with its dysfunction.
