Analytical Data
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Gene name
Claudin-18/CLDN18.2 Protein-VLP
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简介
Claudin-18/CLDN18.2 Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
- Application
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Alternative Names
CLDN18; CLDN18.2; Claudin-18.2; Claudin-18; DKFZp564B2062; SFTA5; SFTPJ
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P56856-2
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Expression Region
M1-V261
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AA Sequence
MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
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Protein Length
Full Length of Isoform A2
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Claudin-18 (CLDN18) is a tight junction protein that plays a critical role in maintaining epithelial barrier function and cell polarity. Its expression is predominantly observed in gastric epithelial cells, making it a significant marker for gastric adenocarcinomas, particularly the CLDN18.2 isoform. The overexpression of CLDN18.2 in various gastric cancer types has drawn attention to its potential as a target for immunotherapy. Recent research has focused on developing recombinant protein-based vaccines and therapeutic agents aimed at eliciting a robust immune response against CLDN18.2-expressing tumors. Virus-like particles (VLPs) serve as an effective platform for displaying antigens due to their ability to mimic natural viruses while being non-infectious and highly immunogenic. By utilizing CLDN18.2 as a target antigen in VLP systems, studies aim to enhance the immune response by presenting the antigen in its native conformational context, thereby improving T-cell activation and potential tumor destruction. The exploration of CLDN18/CLDN18.2 VLP recombinant proteins not only provides insights into targeted cancer therapies but also underscores the promise of VLP technology in developing next-generation cancer vaccines, ultimately contributing to improved patient outcomes in gastric cancer treatment.
